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Harness the power of highly spherical gold nanoparticles in your diagnostics

Over 100 million rapid diagnostic tests use Nano Flow Gold.

High
stability

Our gold manufacturing process produces highly spherical nanoparticles with no angular faces. It delivers an extremely well-dispersed and stable colloidal solution. This prevents the gold particles and gold conjugates from premature aggregation during storage.

High
sensitivity

Our antibody-gold conjugates are prepared according to a proven and unique optimized procedure. Each gold nanoparticle is instantly coupled to the same quantity of antibody, limiting gold aggregation during the coupling process and improving the availability of antibodies on the gold surface for maximum sensitivity.

Batch-to-batch
consistency

Our gold products are manufactured using an automated manufacturing process with state-of-the-art equipment. Integration of in-line control of critical process parameters reduces the risk of human error. Identification of any problems during the manufacturing process also prevents the risk of non-compliant lots.

Use of non-spherical, aggregated purple gold nanoparticles impacts your business as batches are unstable and inconsistent.

High-quality Nano Flow Gold particles and antibody gold conjugates deliver performance you can rely on.

Using poor quality gold nanoparticles has a huge adverse effect on your business and the performance of your diagnostic assays.

Aggregation of gold nanoparticles is a limiting factor in the use of nanoparticles and conjugates over time. This increases the production costs of your diagnostic assays.

Working with non-spherical or quasi-spherical particles also affects your assay performance. Inhomogeneous shapes favor aggregation after antibody conjugation, consequently leading to poor sensitivity and limited dynamic range.

At Nano Flow, we manufacture highly spherical 40nm gold nanospheres, an ideal labeling system for lateral flow and flow-through immunoassay applications.

These particles give gold conjugates :

a bright and highly contrasting ruby color
long-term storage stability due to limited aggregated forms
high sensitivity in your assay format thanks to perfectly dispersed antibody-gold particles.

Our state-of-the-art equipment and automated manufacturing process ensure efficient batch-to-batch reproducibility and decrease the risk of non-compliant products.

Quality Management System certification

iso-9001

Design, development, manufacture and sales of diagnostic reagents, kits and devices for human and animal health and the food industry.

They rely on us

40 nm gold nanoparticles

Nano Flow produces and commercializes 40nm citrate-stabilized gold nanoparticles specifically adapted and optimized for immunochromatographic assays. Our gold particles have highly spherical shapes with no angular faces in contrast to other gold nanoparticles available on the market.

Their unique sphericity limits aggregation and gives gold colloids a bright ruby color and long-term storage stability. Use of Nano Flow Gold colloids simplifies development of rapid diagnostic assays with high sensitivity and a high dynamic range.

Custom gold conjugates

Nano Flow offers a service for antibody/protein custom conjugation to its highly spherical 40nm citrate-stabilized gold nanoparticles. The resulting gold conjugates have high sensitivity and high stability allowing you to develop high-performance and reliable diagnostic assays.

Our innovative industrial process for antibody conjugation ensures successful conjugation. The crucial properties of the gold nanoparticles (polydispersity, color, stability) are not affected during this step, which means our conjugates have maximum activity. Automation of the conjugation process allows for excellent batch-to-batch reproducibility and limits the risk of non-compliant batches.

Custom gold conjugates

Nano Flow offers a service for antibody/protein custom conjugation to its highly spherical 40nm citrate-stabilized gold nanoparticles. The resulting gold conjugates have high sensitivity and high stability allowing you to develop high-performance and reliable diagnostic assays.

Our innovative industrial process for antibody conjugation ensures successful conjugation. The crucial properties of the gold nanoparticles (polydispersity, color, stability) are not affected during this step, which means our conjugates have maximum activity. Automation of the conjugation process allows for excellent batch-to-batch reproducibility and limits the risk of non-compliant batches.

Off-the-shelf conjugates

All our off-the-shelf gold conjugates are produced at a high optical density for ease of use and integration into your platform. All conjugates are designed to deliver maximum sensitivity in diagnostic assays. Use our off-the-shelf conjugates listed below to quickly start your own development and bring your products to market earlier.

  • Goat Anti-Mouse-gold
  • Goat Anti-Chicken-gold
  • Goat Anti-Rabbit-gold
  • Mouse Anti-Rat-gold
  • Rabbit Anti-Goat-gold
  • Mouse Anti-Biotin-gold
  • Mouse Anti-Digoxygenin-gold
  • ProtA-gold
  • ProtG-gold
  • BSA-gold

Nano Flow is both a young and experienced company- young by name, experienced thanks to its history.

  • Nano Flow incorporates 10 years of industrial gold nanoparticle manufacturing, formerly under the umbrella of Unisensor, and the supply of gold for production of over 100 million diagnostic rapid tests.
  • Nano Flow, located in Belgium, has developed a unique and reliable manufacturing process for its highly spherical gold nanoparticles and conjugates.
  • Nano Flow Gold is used in the development of diagnostic rapid assays in the medical, veterinary, environmental and food industries.
  • Using Nano Flow Gold and antibody gold conjugates guarantees maximum performance of diagnostic assays in terms of sensitivity, stability and consistency.

F.A.Q.

How do I order Nano Flow products ?

Placing an order is simple: contact us via the contact form on our website to describe your needs. We will then get back to you for more information or send you a quote within 3 workdays. If accepted, you can send your purchase order to info@nanoflow.eu. We will plan activities and keep you informed on progress and delivery.

I have not heard of Nano Flow before. How can I trust your products ?

“Nano Flow” is a new name for 10+ years of business and experience in gold nanoparticle and antibody conjugate manufacturing at Unisensor, a pioneer in diagnostic engineering. The gold in Nano Flow has already been used in more than 100 million rapid tests with applications in medical, veterinary, environmental and food diagnostics. Our automated manufacturing process complies with quality standards in accordance with our ISO9001:2015 certification and is audited each year by certification authorities.

Why have my gold particles settled at the bottom of the bottle? Can I still use them ?

The settling of gold nanoparticles is a normal phenomenon associated with gravity. This phenomenon is amplified when using bigger particles in aqueous solutions. Settling does not affect the performance of our nanoparticles. Before use, we recommend swirling the solution to properly disperse the nanoparticles and yield a homogeneous solution. The expiration dates for our gold nanoparticles are longer than 1 year.

How should I store gold particles and gold conjugates ?

Gold nanoparticles and antibody gold conjugates must be stored between 2 and 8°C, protected from light.

I want to conjugate Nano Flow Gold particles by myself. How do I proceed ?

Coupling antibodies or proteins to a gold surface by passive adsorption is fairly easy, at least at small scale. First, you need to determine the optimal coupling pH by screening pH values from 6 to 9.

The second step consists of finding the right antibody or protein concentration to stabilize your gold nanoparticles and avoid aggregation. Protein concentrations in the range of 3 to 5 µg/ml/OD usually work well. The stability of your gold conjugates can be estimated and compared by addition of Sodium Chloride to the medium and subsequent observation of the aggregation tendency of the conjugate.

The optimal antibody or protein concentration is the one that does not result in, even after adding salts, change to the ruby color of the gold particles. If you need more information, please contact us. We will help you to ensure the success of your conjugate development.

Contact details

Nano Flow
Rue Louis Plescia, 8
4102 Ougree (Liege)
Belgium

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